After a 20-min incubation with immunol staining blocking buffer (Beyotime, Shanghai, China), cells were continuously incubated with mouse anti-SMA (1:100, Abcam) and rabbit anti-CD133 (1:100, Abnova) 44 at 4 C overnight
Pico Toning Delivers low-energy laser across the entire face for overall brightening and even skin tone
Primary mouse peritoneal macrophages, mouse macrophage-like RAW 264.7 cells, and human monocytic THP-1 cells were transfected with GSTP encoding expression vector (pcDNA3.1-Flag-GSTP) or empty vector (pcDNA3.1), followed by stimulation of LPS (500 ng/ml) for 18 h, and the levels of HMGB1 in the culture medium were determined by ELISA
Markers of oxidative stress and antioxidant enzyme activity were quantified, including total hydroperoxides (THP), malondialdehyde (MDA), superoxide dismutase (SOD), and catalase