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Evans-blue vascular permeability assay To assess vascular permeability, animals (n=9-9) received 50 mg/kg Evans blue (Reanal, Hungary, dissolved in physiological saline at a concentration of 2.5%) via a femoral venous catheter

Especially for animals with an overburdened liver, animals that take medication for a long time or recover from anesthesia, and older animals, because the own production of glutathione decreases while the need increases