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STUDY DESIGN The study was a double-blind parallel placebo-controlled randomized clinical trial

Community support, accountability partners, or formal behavior change programs all show strong evidence for improving long-term maintenance

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Lipsky and Pagano devised a way to increase probe uptake by incubating cells at 4 C to block endocytosis while permitting probe adhesion to the plasma membrane, followed by rapid temperature increase to 37 C to activate endocytic pathways