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Sample or standard was added to GCL reaction buffer (400 mM Tris, 40 mM ATP, 20 mM L-glutamic acid, 2.0 mM EDTA, 20 mM sodium borate, 2 mM serine, and 40 mM MgCl 2 )

Clinical studies show that patients maintaining thigh muscle mass during weight loss report up to 30% less knee pain progression than those experiencing significant muscle loss

These effects are most likely due to IGF-1-induced increased calcitriol synthesis, resulting in enhanced intestinal and renal calcium absorption ( vide supra )

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