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2 2.8.3 Widely targeted metabolomics analysis Frozen tissue specimens were homogenized and extracted using ice-cold methanol:water (7:3, v/v) supplemented with isotope-labeled internal standards,followed by multi-platform LC-MS/MS analysis
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In their discussion the researchers stated that exposure to heavy metals increased abundances of Firmicutes, Proteobacteria and Bacteroidetes in females, and speculate that the relative protection seen in the female fish may be due to the reported link between the Firmicutes such as Lachnoclostridium-10 , Ruminococcaceae, and Lactobacillus and SCFAs, which can enhance intestinal endocrine function, prevent infection, and protect intestinal health (Lemaire et al., 2018)
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