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Blots were blocked in either 5% (w/v) nonfat milk (Lab Scientific, M-0841) or 1% (w/v) BSA in 1 Tris-buffered saline with Tween (TBST) (Teknova, T1688) for 3060 min at room temperature, incubated with primary antibodies in TBST for 1 h at room temperature, washed three times with TBST for 5 min each, incubated with fluorophore-conjugated secondary antibodies for 30 min at room temperature, washed three times with TBST and imaged on an Odyssey CLx imager (LI-COR) or ChemiDoc XRS+ imager with Clarity western enhanced chemiluminescence blotting substrates (Bio-Rad)

[5] GHK-Cu and NAD+ are not competitors: GHK-Cu targets structural repair in the skin and connective tissue NAD+ targets energy production and cell maintenance across the entire body Using both makes sense if both goals are relevant

This is where the lesser occipital nerve travels, and it also happens to be the third spot normally done for an occipital nerve block

S., Rong Ling, J