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The substantial impact of LASSS pre-treatment was more pronouncedly demonstrated by an alternative experiment using the disulfide lipoic acid (LA, containing two sulfur atoms connected by a disulfide bond)

doi: 10.1016/j.fertnstert.2008.10.058 163

Such GPX4-independent surveillance pathways are often mediated by enzymes that generate metabolites with radical-trapping antioxidant (RTA) activity, including ferroptosis suppressor protein 1 (FSP1), dihydroorotate dehydrogenase (DHODH), and GTP cyclohydrolase-1 (GCH1), etc 10,14,15,16

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