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The small second-order rate constant for the simulated reaction between GSH and the complex of reduced PfGrx C88S and roGFP2 WT (S 2 ) might reflect a poor accessibility of the 1st glutathione interaction site in this complex (Fig

The supernatants were lyophilized and derivatized with methoxyamine hydrochloride, pyridine, and N-methyl-N-(trimethylsilyl)trifluoroacetamide, before being analyzed by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS)