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For experiments in 384-well plates, 500 cells were seeded per well, and after 24 h, the medium was switched using a series of 4 aspirationwash steps with the desired final medium using Multidrop Combi (Thermo)

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It missed its main target too, but a post hoc slice of the data, the patients taking fosgonimeton on its own without a standard Alzheimers drug alongside it, showed a striking signal on a brain-wave measure called P300 latency, a marker of processing speed, that moved 28 milliseconds in the right direction, along with a hint on a cognitive scale (ACT-AD topline, June 2022)

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