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Hit compounds identified from the high-throughput screen were validated via dose-response inhibition assays and further evaluated for cytotoxicity in MCF-7 estrogen and progesterone receptor-positive breast cancer cells and MDA-MB-231 triple-negative breast cancer cells

[8] Very high doses may occasionally cause hypokalaemia (low potassium), particularly in patients with severe megaloblastic anaemia when treatment is initiated, necessitating monitoring in hospital settings