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2.4 Sample size calculation The study was designed to include 16 eyes (8 in each group), providing a 95% statistical power to detect a significant difference between the hyaluronic acid + glutathione group and the hyaluronic acid-only group, assuming a 20% dispersion index and a two-sided = 0.05 ( 2.5 Study procedures All tests and data collection were performed by a cornea specialist