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Our results demonstrated a significant downregulation of the PI3K/AKT pathway ( p = 0.049) at one hour, and of mTOR ( p = 0.033 at one hour and p = 0.021 at two hours) following APAP treatment in wild-type mice (Fig

The authors explicitly frame their own finding as relevant to the injection question: topical application, they note, "may offer an effective alternative to injection," comparing the quantity of copper made bioavailable through a realistic topical patch against a reference subcutaneous injection dose used in animal research

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