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This indicated that the TrxR1/Gsr-null livers excrete much more oxidized GSSG than do wild type livers, which likely is critical for sustaining the reduced state of the intracellular [GSH/GSSG] redox couple in the absence of GSSG-reductase activity [107]

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Most of the commonly used oxidoreductase assays for glutaredoxins are either discontinuous and labor-intensive or unidirectional and coupled to the glutathione reductase-dependent consumption of NADPH 1,2,6,8,16,17,18,30