Pay in 4 interest-free payments of $7.18 Learn more
Ships within 48 hours · Estimated delivery Aug 11 - Aug 16
Biological sample collection After 3 weeks of treatment, all mice were anesthetized with isoflurane (35%), blood was collected from the abdominal vena cava
Hot-spot KIF5A mutations cause familial ALS
Another dCas9 fusion is the synergistic activation mediator (SAM) system, which consists of three total components: dCas9 fused to VP64 with a nuclear localization sequence (NLS) (NLS-dCas9-VP64), two activation domains (p65 and human heat-shock factor 1 (HSF1)) fused to the MS2 bacteriophage coat protein (MS2-p65-HSF1), and the sgRNA fused to two MS2 protein-targeting hairpin aptamers at the stem-loop 2 and tetraloop regions (sgRNA2.0) [70]
Briefly, the gene encoding the OsDHAR sequence (residues 2203) was amplified by PCR using Ex Taq polymerase (TaKaRa Bio Inc., Shiga, Japan) with forward and reverse primers for a cDNA template (Table S1)
US$ 28.55
US$ 21.45
US$ 22.00
US$ 25.71
US$ 20.94
US$ 23.93
US$ 28.76
US$ 27.03
US$ 25.35
US$ 21.08
US$ 20.01
