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When a tumor develops, the host spontaneously elicits an immune response to suppress the growth of tumor cells ( in vivo , tumor cells have developed various methods to avoid the recognition and attack by the immune system, which is called immune evasion ( In the adaptive immune system, there are T cells (CD4 + , CD8 + T, and NKT cells), B cells, and plasma cells in TME

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To determine how the production and utilisation of betaine may be affected, the betaine: choline and DMG: betaine ratios were also investigated