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Tube formation assay The extracellular matrix gel-induced tube formation assay was used as an in vitro measurement of angiogenesis

Briefly, L-lysine (0.78 g, 5 mmol) and D, L-Hcy thiolactone hydrochloride (0.93 g, 5 mmol) were dissolved in 100 mL 0.2 M sodium phosphate buffer, pH 7.4, 0.2 mM EDTA and allowed to react for 24 h at room temperature