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Library preparation was done by using the Illumina Stranded mRNA Library Preparation kit, with 100 ng of total RNA as starting material

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Without enough, you might get megaloblastic anemia , with big, abnormal red blood cells

To identify newer evidence, a search was conducted on PubMed for randomised clinical trials (RCTs), systematic reviews and meta-analyses published in English between 1 January 2014 and 28 February 2018