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10.1016/j.jare.2024.01.035 95 LianM

Samples were resuspended in 160 L EPPS buffer (200 mM, pH 8.0) using a probe sonicator (1 1015 pulses, power output 20%) and then digested with LysC (4 L of 0.5 g/L per sample, resuspended in HPLC grade water, Wako-chemicals, Fujifilm #125-05061) for 2 h at 37 C in a shaker incubator

This range of the pore sizes in the beads defines the fractionation range of a column, and within this range, molecules are eluted in order of decreasing size