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The isolated metatarsals were cultured in 24-well culture plates in 150 L of DMEM supplemented with 10% heat-inactivated fetal bovine serum and 1% of penicillin/streptomycin and 1% L -glutamine for 72 h

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55 For efficient tumor antigen processing and presentation to T cells, activated DCs need high rates of cell surface or secretory protein biosynthesis, which is partly regulated by FAS-induced increases in cytokine production

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