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Cells were then lysed in 1X TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.4) supplemented with 1 mM EDTA, 1% Triton X-100, phosphatase inhibitor cocktail (P5726, Sigma Aldrich), and protease inhibitor cocktail (11873580001, Roche Diagnostics)
These patents were originally filed as an international application on March 20, 2006
Once this vial has been open, it has been exposed to air and germs
Dayan, [email protected] These authors have contributed equally to this work Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers
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