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Description

For fixation, an equal volume of PBS (Thermo Fisher Scientific) + 8% paraformaldehyde (Electron Microscopy Sciences) was added to the medium for 15 min, after which the samples were washed three times with PBS

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[11] Heavy bleeding that fills the mouth or throat, causes difficulty breathing, or results in significant blood loss necessitates emergency evaluation

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Tried everything out there

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2019), with UMI threshold calculated adaptively per sample based on the mean and standard deviation of the background counts distribution

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