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Ketogenic therapies in Parkinson's disease, Alzheimer's disease, and mild cognitive impairment: an integrative review

The elevated growth hormone then boosts IGF-1 production in the liver and other tissues, which mediates many of GHs anabolic effects including increased protein synthesis and muscle growth, enhanced lipolysis and fat body function, improved bone density and tissue repair, enhanced collagen synthesis, and better sleep quality and healing

Ellison S, Parker H, Bigger B

Samples were then precipitated with 1 mL absolute MeOH at 40 C for 2 h, centrifuged (16,000 g at 0 C), and the obtained supernatants was evaporated and the residue was resuspended and sonicated in 100 L 50 mM HEPES buffer (pH 7.4)