Non-response to semaglutide occurs in roughly 5-10% of patients and often reflects genetic factors limiting GLP-1 receptor activation
We used 50 ng of cDNA for the PCR template, real-time PCR was conducted using thermal cycler Quant Studio 3 (Thermo Fisher Scientific, Waltham, MA, USA) and thermal cycling conditions were: 2 min at 50C and 10 min at 95C followed by 40 cycles at 9C for 15 s and at 60C for 1 min (37)
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Currently, there are two anti-PCSK9 monoclonal antibodies, alirocumab and evolocumab, that have been FDA-approved for their use in the management of hypercholesterolemia, and other PCSK9-targeting strategies, including anti-PCSK9 vaccination, genome editing, the use of siRNA, and more (Sun et al., 2022)
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