Two expert physicians were designated to act as the guideline coordinators and their functions were: 1 To carry out a thorough search of the literature on the Pubmed, Embase, Medline, Trip Database, Clinical Evidence, and Cochrane Library databases, to compile all current and relevant information
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In brief, chemical standard libraries of 149 metabolites in seven pooled libraries were prepared and serially diluted in high-performance liquid chromatography (HPLC)-grade water from in a dilution series from 5 mM to 1 M to generate external standard pools, which are used for calibration of isotopically labelled internal standards and to quantify concentrations of metabolites for which internal standards were not available
