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Corticotropin-releasing hormone (CRH) and arginine vasopressin (AVP) secreted by the hypothalamus act on the anterior pituitary and accelerate the release of adrenocorticotropic hormone (ACTH) and its stimulation of the adrenal cortex, further triggering the secretion of cortisol and other hormones and ultimately reaching the target site through blood circulation, affecting the functional and immune states of the intestinal tract (111)

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In the context of a restricted assay-format, in which implication of free LASSS can be excluded, the outcomes may provide critical insights towards understanding of an underlying LASSS-driven mechanism